Journal of Dental Sciences and Oral Hygiene – Online First
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Article (s)
Volume 1, Issue 1 J dent Sci Oral Hygiene, 2023
Efficacy of Mechanical, Subsonic, Ultrasonic and Photon-Induced Photoacoustic Streaming techniques in reducing Enterococcus faecalis in severely curved root canals: An ex vivo study
Osvaldo Zmener, Cornelis H Pameijer and Roberto Della Port
The aim of the study was to compare in vitro the efficacy of Photon-induced Photoacoustic Streaming (PIPS) with EndoActivator (EA), Ultrasound (PUI), XP-endo Finisher (XP) and conventional needle irrigation (CNI) in reducing Enterococcus faecalis from severely curved root canals. Fifty-four extracted human mandibular premolars with 35-45 degree canal curvature were prepared and irrigated with 5.25% sodium hypochlorite and 17% EDTA. After preparation the canals were autoclaved and then inoculated with E. faecalis. Fifty teeth were assigned to 5 experimental groups (n=10 each), while 4 teeth were used as positive and negative controls (n=2 each). In the experimental groups, the inoculated canals were irrigated with 5.25% sodium hypochlorite and the solution agitated with EA, XP, PUI or PIPS. In Group 5, the canals were irrigated without agitation using the CNI technique. Samples of canal contamination were obtained before and after irrigant agitation using sterile paper points. The infected material was vortexed in sterile saline. After serial dilutions, aliquots were plated on blood agar plates and incubated for 30 days. Microbial reduction/removal in the canals was determined by counting colony formed units (CFUs). Data was analyzed by the Kruskal-Wallis and Dunn´s multiple comparison test. Significance was established at P<0.05. The positive controls presented high concentration of CFUs. The negative controls showed no bacterial growth. There were no significant differences (P>0.05) between EA, XP, PUI and PIPS groups in their antimicrobial efficacy against E. faecalis but there was a significant difference (P<0.05) with the CNI Group. Although EA, XP, PUI and PIPS were comparable in their ability to reduce E. faecalis from severely curved root canals none of them including the CNI samples were capable of effectively eliminating the total microbial load.
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