Pathology and Pathophysiology

All the manuscripts published by ‘Pathology and Pathophysiology’ undergo rapid, quality, and quick review processing by eminent editorial and review teams maintaining high standards and ethics of publishing. The scholarly content published online will be freely available to every reader anywhere in the world to read, download, copy, reuse and distribute, provided that the original work is properly cited.

Article (s)

Volume 1, Issue 1  Pathol Pathophysiol, 1:1

Anti-JOVI.1 Antibody to Detect Clonal T Cell Populations: Implementation into a Diagnostic Flow Cytometry Laboratory and Correlation with Clinical Findings

Benjamin Reardon*, Jennifer Hsu, Sandy Smith, Riana van der Linde, David A. Brown,  Elizabeth Tegg, and Sarah C. Sasson


The development of a high through-put flow cytometric assay for the identification of clonal T cells has proved challenging. We assessed the surface expression of a specific T Cell Receptor β-chain constant region using conjugated anti-JOV1.1 monoclonal antibodies to identify clonal T cell populations in a large diagnostic flow cytometry laboratory within a quaternary referral hospital. 37 cases were analysed. We identified 15 cases of clonal JOVI.1 expression, 7 of which had a consensus diagnosis of T-cell lymphoproliferative disease (TLPD). The remaining 22 cases had polyclonal JOVI.1 expression, none of which had a consensus diagnosis of TLPD, resulting in a sensitivity of 100% and specificity of 73%. When clonal NK-T cells were excluded, specificity further improves to 97%. These results provide real-world data and support the widespread adoption of this assay into diagnostic use.


Full Text

Supplementary Files